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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
02/03/2017 |
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Actualizado : |
07/03/2017 |
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Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
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Idioma : |
Inglés |
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Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02512naa a2200217 a 4500
001 1056750
005 2017-03-07
008 2015 bl uuuu u00u1 u #d
100 1 $aGARCIA, J.P
245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource]
260 $c2015
520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease.
650 $aENFERMEDADES DE LOS ANIMALES
700 1 $aGIANNITTI, F.
700 1 $aFINNIE, J.W.
700 1 $aMANAVIS, J. .
700 1 $aBEINGESSER, J.
700 1 $aADAMS ,V.
700 1 $aROOD, J.I.
700 1 $aUZAL, F.A.
773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
22/03/2016 |
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Actualizado : |
28/09/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CLARIGET, J.; ROMAN, L.; KARLEN, M.; ÁLVAREZ-OXILEY, A.; LÓPEZ-MAZZ, C.; PÉREZ-CLARIGET, R. |
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Afiliación : |
JUAN MANUEL CLARIGET BRIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LORENA CAROLINA ROMAN GAY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAURICIO KARLEN, Universidad de la República (UdelaR)/ Facultad de Agronomía; ANDREA ÁLVAREZ-OXILEY, Universidad de la República (UdelaR)/ Facultad de Agronomía; CARLOS LÓPEZ-MAZZ, Universidad de la República (UdelaR)/ Facultad de Agronomía; RAQUEL PÉREZ-CLARIGET, Universidad de la República (UdelaR)/ Facultad de Agronomía. |
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Título : |
Supplementation with a mixture of whole rice bran and crude glycerin on metabolic responses and performance of primiparous beef cows. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Revista Brasileira de Zootecnia, 2016, v. 45, no.1.p.16-25. |
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ISSN : |
1806-9290 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received April 17, 2015 //Accepted November 18, 2015. |
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Contenido : |
ABSTRACT - This study investigated the effect of a supplement containing whole rice bran and crude glycerin for
21 days before mating on metabolic, productive, and reproductive responses of 28 primiparous suckling beef cows. Cows
were randomly assigned to a control group (CON, n = 14), grazing on grasslands, and a supplemented group (SUP, n = 14),
grazing on grasslands and supplemented daily individually with 1 kg dry matter (DM) of whole rice bran + 550 mL crude
glycerin (224 g kg?1 DM of methanol) per cow. After 33 days of natural mating, cows that had not expressed estrus were
subjected to a fixed-time artificial insemination protocol. Ten days after the insemination program, bulls were reintroduced for
21 days. Supplementation increased milk yield (SUP: 5.7±0.2 vs. CON: 5.0±0.2 kg d?1 ), milk protein content (SUP: 3.1±0.2
vs. CON: 2.8±0.2%), and body weight of cow (SUP: 379±2 vs. CON: 373±2 kg) and calf (SUP: 150±2 vs. CON: 142±2 kg).
Supplementation improved the energy balance, increased plasma concentrations of cholesterol (SUP: 223.2±6.4 vs. CON:
202.1±6.4 mg dL?1) and glucose (SUP: 72.0±1.2 vs. CON: 68.6±1.2 mg dL?1), and reduced non-esterified fatty acids
(SUP: 0.45±0.02 vs. CON: 0.56±0.02 mmol L?1 ). The percentage of cows on superficial anestrous after supplementation was
greater in SUP than in CON group (57 vs. 21%, respectively); however, no difference in final pregnancy rate was found
(SUP: 79 vs. CON: 64%). There was no evidence that the ingestion of crude glycerin with high content of methanol induced
clinical or hepatic disorders. Supplementation of whole rice bran and crude glycerin is not toxic, and can improve the energy
balance, reflecting in increase in milk yield and calf growth, with a slight effect on the reproductive activity- MenosABSTRACT - This study investigated the effect of a supplement containing whole rice bran and crude glycerin for
21 days before mating on metabolic, productive, and reproductive responses of 28 primiparous suckling beef cows. Cows
were randomly assigned to a control group (CON, n = 14), grazing on grasslands, and a supplemented group (SUP, n = 14),
grazing on grasslands and supplemented daily individually with 1 kg dry matter (DM) of whole rice bran + 550 mL crude
glycerin (224 g kg?1 DM of methanol) per cow. After 33 days of natural mating, cows that had not expressed estrus were
subjected to a fixed-time artificial insemination protocol. Ten days after the insemination program, bulls were reintroduced for
21 days. Supplementation increased milk yield (SUP: 5.7±0.2 vs. CON: 5.0±0.2 kg d?1 ), milk protein content (SUP: 3.1±0.2
vs. CON: 2.8±0.2%), and body weight of cow (SUP: 379±2 vs. CON: 373±2 kg) and calf (SUP: 150±2 vs. CON: 142±2 kg).
Supplementation improved the energy balance, increased plasma concentrations of cholesterol (SUP: 223.2±6.4 vs. CON:
202.1±6.4 mg dL?1) and glucose (SUP: 72.0±1.2 vs. CON: 68.6±1.2 mg dL?1), and reduced non-esterified fatty acids
(SUP: 0.45±0.02 vs. CON: 0.56±0.02 mmol L?1 ). The percentage of cows on superficial anestrous after supplementation was
greater in SUP than in CON group (57 vs. 21%, respectively); however, no difference in final pregnancy rate was found
(SUP: 79 vs. CON: 64%). There was no evidence that the ingestion of crude gly... Presentar Todo |
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Palabras claves : |
GLICERINA CRUDA; METHANOL; MILK PROTEIN; PROTEÍNA DE LECHE; SALVADO DE ARROZ; SHALLOW ANESTRUS; VACA DE CARNE. |
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Thesagro : |
METANOL. |
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Asunto categoría : |
L52 Fisiología Animal- Crecimiento y desarrollo |
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Marc : |
LEADER 02745naa a2200301 a 4500
001 1054496
005 2018-09-28
008 2016 bl uuuu u00u1 u #d
022 $a1806-9290
100 1 $aCLARIGET, J.
245 $aSupplementation with a mixture of whole rice bran and crude glycerin on metabolic responses and performance of primiparous beef cows.$h[electronic resource]
260 $c2016
500 $aArticle history: Received April 17, 2015 //Accepted November 18, 2015.
520 $aABSTRACT - This study investigated the effect of a supplement containing whole rice bran and crude glycerin for 21 days before mating on metabolic, productive, and reproductive responses of 28 primiparous suckling beef cows. Cows were randomly assigned to a control group (CON, n = 14), grazing on grasslands, and a supplemented group (SUP, n = 14), grazing on grasslands and supplemented daily individually with 1 kg dry matter (DM) of whole rice bran + 550 mL crude glycerin (224 g kg?1 DM of methanol) per cow. After 33 days of natural mating, cows that had not expressed estrus were subjected to a fixed-time artificial insemination protocol. Ten days after the insemination program, bulls were reintroduced for 21 days. Supplementation increased milk yield (SUP: 5.7±0.2 vs. CON: 5.0±0.2 kg d?1 ), milk protein content (SUP: 3.1±0.2 vs. CON: 2.8±0.2%), and body weight of cow (SUP: 379±2 vs. CON: 373±2 kg) and calf (SUP: 150±2 vs. CON: 142±2 kg). Supplementation improved the energy balance, increased plasma concentrations of cholesterol (SUP: 223.2±6.4 vs. CON: 202.1±6.4 mg dL?1) and glucose (SUP: 72.0±1.2 vs. CON: 68.6±1.2 mg dL?1), and reduced non-esterified fatty acids (SUP: 0.45±0.02 vs. CON: 0.56±0.02 mmol L?1 ). The percentage of cows on superficial anestrous after supplementation was greater in SUP than in CON group (57 vs. 21%, respectively); however, no difference in final pregnancy rate was found (SUP: 79 vs. CON: 64%). There was no evidence that the ingestion of crude glycerin with high content of methanol induced clinical or hepatic disorders. Supplementation of whole rice bran and crude glycerin is not toxic, and can improve the energy balance, reflecting in increase in milk yield and calf growth, with a slight effect on the reproductive activity-
650 $aMETANOL
653 $aGLICERINA CRUDA
653 $aMETHANOL
653 $aMILK PROTEIN
653 $aPROTEÍNA DE LECHE
653 $aSALVADO DE ARROZ
653 $aSHALLOW ANESTRUS
653 $aVACA DE CARNE
700 1 $aROMAN, L.
700 1 $aKARLEN, M.
700 1 $aÁLVAREZ-OXILEY, A.
700 1 $aLÓPEZ-MAZZ, C.
700 1 $aPÉREZ-CLARIGET, R.
773 $tRevista Brasileira de Zootecnia, 2016$gv. 45, no.1.p.16-25.
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